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Peptide HIV/AIDS Treatment

Last Updated on Sunday, 15 November 2009 23:40

Peptide Immunotherapy

A new direction in HIV/AIDS treatment

by Andrew Keech, PhD1, Uche Nwabuko, MD2,  Chikezie Chikezie, MD3, G.U.E. Wogu, MD3

1 Advanced Protein Systems, Phoenix, AZ  2 Enugu State Hospital, Enugu, Abia State, Nigeria  3UDECO Medical Centre, Suite E100, Ikota Shopping Complex, Km 22, Lagos-Epe Expressway. Victoria Garden City, Lekki, Nigeria

ABSTRACT

A balanced immune system is critical in maintaining good health. Proline-Rich Polypeptides (PRP) are known to have the ability to both up-regulate and down-regulate the immune system.  As PRP is available in abundant quantities from bovine colostrum and at low cost, several independent a research trials using an oral PRP vanilla flavored mouth spray were designed to test its effectiveness for HIV/AIDS patients, particularly those in areas of the world where Anti-Retroviral (ARV) Treatments are unavailable.  Preliminary phase I studies showed promise in increasing or normalizing CD4+ T-cell counts in HIV/AIDS patients, so phase II studies were carried out in Kenya and Nigeria with more patients.  Results of the studies showed increases in CD4+ T-cell counts to normal or near normal levels, reduction in viral loads, and the remission of HIV/AIDS related physical symptoms in most patients with days, reduction of clinical symptoms, as well as significant weight gains, within six to twelve weeks of treatment.  This suggests that PRP spray may prove to be a useful, low cost, easy to use, and side effect-free adjunct therapy in the treatment of HIV/AIDS.  PRPs are shown to be increasingly popular and well tolerated immune and cytokine modulators for both immune suppressed and auto-immune conditions.


Background

The appearance of HIV/AIDS in the early 1980s forced medical science to reevaluate itself in regard to infectious disease in general and viral infections in particular.   Prior to this time  it appeared that modern medicine had infectious disease on the run.  Age-old killers like polio and smallpox had been all but eliminated.  There was a general feeling that all infectious diseases would be conquered in time.

HIV/AIDS, however, graphically demonstrated that our knowledge of viruses and  how to treat viral infections was horribly lacking.  Our ignorance of the retrovirus that finally was linked to the disease and our futile attempts to control it compelled new approaches and ways of thinking.

In the quarter century since HIV/AIDS first appeared, a great deal has been learned about viruses in general and retroviruses in particular.  Many new treatments have emerged for both HIV infection itself and the opportunistic diseases which take advantage of the compromised immune systems of HIV/AIDS sufferers.  Yet still the cure for the disease eludes us, as does an effective vaccine.  According to the Joint United Nations Programme on HIV/AIDS and the World Health Organization (WHO), some 25 million people have died of HIV/AIDS in those 25 years (that's a million a year), and an estimated 38.6 million are infected with the virus, making it one of the most lethal epidemics in history.  In 2005, HIV/AIDS claimed 2.4-3.3 million lives, including over 570,000 children[i].

While sub-Saharan Africa has been hardest hit, HIV/AIDS is a major problem globally.  Over one million are reported to be infected in China and six million in India.  More than a half million have died from HIV/AIDS in the US, and over a million are infected[ii]. In Botswana, roughly one in three people in the entire country are infected, and life expectancy has declined from 65 pre-HIV/AIDS to only 40 today[iii].

Efforts continue to find both a cure for HIV/AIDS and an effective vaccine to prevent further HIV/AIDS infections.  Yet the very nature of retroviruses make them an exceedingly difficult target.

HIV is a single-stranded, positive-sense, enveloped RNA virus.  When the virus infects a cell, its RNA is encoded into a double-stranded DNA molecule by a virally encoded reverse transcriptase molecule present in the viral particle.  The viral DNA is then integrated into the cellular DNA by a virally encoded integrase enzyme.  Often the virus will become latent at this stage, making any antiviral treatment impossible until it once again becomes active.  This latency period can last for years.   When the virus becomes active, it replicates and produces large numbers of viral particles that are then released to infect other cells.

What is particularly lethal about HIV is that it primarily infects the very cells in the immune system that would normally keep it in check – CD4+ T cells, macrophages and dendritic cells.  Infection of CD4+ cells kills in three different ways: direct viral killing of the cells; increased rates of apoptosis (programmed cell death) in infected cells; and targeting of CD4+ cells by CD8 cytotoxic lymphocytes that recognize infected cells and destroy them.  This loss of CD4+ cells is cumulative, and eventually the numbers of CD4+ cells decline below critical levels to where cell-mediated immune function is lost.  This leaves the body open to opportunistic infections like Pneumocystis pneumonia and Kaposi's sarcoma, which are what actually kill victims.  By robbing the body of its own defenses, HIV ultimately kills its host, though at times over a period of years.  The virus also mutates rapidly making it difficult to produce an effective vaccine.

The main strategy that the scientific community has used in its attempts to attack HIV reflect the trends used against other pathogens, namely a pharmaceutical strategy to directly attack the virus.  As such the antiviral drugs that have been developed to combat HIV have many of the same limitations as previous pharmaceutical drugs developed to combat viral infections.  First, they target the infected cells directly, usually by disrupting their ability to replicate the virus.  Unfortunately, many uninfected cells in the area of the infected cells are collaterally affected and killed.  These drugs are also not effective in all patients.

Secondly, all of the antivirals developed to fight HIV have serious side effects, including nausea, diarrhea, vomiting, anemia, and others.  Finally, these drugs are very expensive and thus not available to those who have no insurance coverage or other means of paying for them.  This is a major problem in Africa where nearly all HIV/AIDS victims have no means to pay for expensive antiretroviral therapies (ART).   Combination therapy, which is currently the treatment of choice, costs about $950 a month.  Drug companies have lowered their prices in some African countries to about $500 a month, but this is still far beyond most people’s ability to pay.  The average monthly salary among middle class wage earners in Uganda, for example, is only about $400 a month[iv].

Currently the FDA has approved 29 pharmaceutical drugs for use in the treatment of HIV infection[v].  Nearly all inhibit viral replication and include reverse transcriptase inhibitors and protease inhibitors.  One, Fuzeon, blocks viral fusion to target cells.  HIV has responded by developing resistant strains that are not affected by the drugs, even combinations of them.  The future outlook for HIV/AIDS treatment from a pharmaceutical perspective remains bleak despite significant gains in understanding the virus.

This situation has forced scientists to look elsewhere for effective solutions.  ART focuses primarily on attacking infected cells directly.  A more effective method would be to stimulate the body’s own defenses to attack the virus as well as infected cells.  This would make it much more difficult for the HIV to avoid attack through mutation as the immune system has the ability to adapt to the new strains rapidly.

Supplementation of many natural occurring compounds claim to boost human immunity by activating human Natural Killer (NK) activity.  These include Shitake Mushrooms, Carotenoids, Garlic, Echinacea, some Oligosaccharides (Ginseng, Arabinoxylane[vi], Arabinogalactin[vii]), Canova[viii], and Superoxyde Dismutase[ix].  Other immune boosting compounds include Vitamin A, C, D, E, Calcium, Zinc, Selenium, Omega-3 fatty acids, Bee Propolis, Bioflavenoids, and many others not listed.  Unfortunately, modern science is quick to dismiss many of these natural immune remedies.  However, they do deserve mention here.

Ironically, modern science is discovering the importance of NK activation in fighting viral conditions like HIV/AIDS, especially among some seropositive prostitutes and drug users[x].  Unfortunately, in some cases un-natural immunomodulatory stimulation of the T-Cell activity may be fatal[xi]

One such area of investigation is based on an old remedy, colostrum, the first milk produced by a mammal following the birth of a newborn, which was widely investigated as an antibiotic before modern antibiotics were developed.  Colostrum and derivatives of it have already demonstrated their usefulness in treating opportunistic infections associated with HIV/AIDS, such as diarrhea caused by Clostridium, Campylobacter and Amoeba spp[xii].  Specifically one of the components of colostrum, called alternatively PRP (proline-rich polypeptide), transfer factor, dialyzable leukocyte extract (DLE), infopeptides, or colostrinin, has shown great promise.  This unique polypeptide (actually a peptide fraction of whole colostrum) has been shown to have immunomodulatory abilities as well as antiviral activity[xiii].

The advantage over other natural remedies listed previously, including colostrum powder supplementation, is a much shorter response time of physical symptoms, relatively quick normalization of NK activity, abundance and low cost of raw colostrum in the world.  The key is the protein fractionation technology needed to perform the PRP extraction.  This has current patents pending[xiv],[xv].

The principal immunomodulatory action of PRP is to stimulate the maturation of immature thymocytes into either helper or suppressor (also called regulatory) T cells[xvi],[xvii], depending on the need of the body at the time.  Helper T cells present antigens (such as a viral protein) to B lymphocytes, which then produce antibodies to that antigen[xviii].  Helper T cells also help produce memory T cells which retain the “memory” of an antigen in order to expedite the production of antibodies in the event the antigen is reencountered in the future[xix].  Suppressor T cells, on the other hand, deactivate other lymphocytes after an infection has been cleared to avoid damage to healthy tissues[xx].  PRP also promotes the growth and differentiation of B cells in response to an infection[xxi] and the differentiation and maturation of macrophages and monocytes[xxii].  The activity of Natural Killer (NK) cells, cytotoxic cells of the innate immune system, was increased up to 5 times by PRP[xxiii],[xxiv],[xxv].

PRP modulates the cytokine system as well.  It stimulates the production of a wide range of cytokines, including the pro-inflammatory cytokines tumor necrosis factor-alpha (TNF-α), which initiates the inflammatory cascade of cytokine production, and interferon-gamma (INF-γ), and the anti-inflammatory cytokines interleukins-6 and -10 (IL-6 and IL-10)[xxvi].

PRP functions as a molecular signaling device which works through receptors on target cell surfaces[xxvii] to initiate or suppress the production of specific proteins.  It is not species specific; PRP from bovine colostrum works as effectively in humans as PRP from human colostrum[xxviii].  As it is a natural product, there are no known side effects or drug interactions, and it can be taken safely by all ages.

Preliminary experimental and clinical studies have shown that PRP holds great promise in combating HIV/AIDS.  In an experimental in vitro system, PRP blocked HIV infection of cells[xxix].  PRP in combination with zidovudine (ZDV), an anti-retroviral drug, is known to be effective in patients suffering from HIV/AIDS-Related Complex (ARC), increasing levels of white blood cells, CD8 lymphocytes and IL-2[xxx].  A preliminary study on 25 men with HIV/AIDS resulted in clinical improvement or a stabilized clinical condition in 20 of the 25.  12 of 14 anergic (unresponsive to antigenic stimulation) patients demonstrated restored delayed type hypersensitivity to recall antigens within 60 days[xxxi].

Recent research has found that while HIV targets both helper CD4+ and suppressor (or regulatory) CD4+ T cells, they are not suppressed at the same rate.  In fact, regulatory T cells decline at a slower rate than helper T cells.  As regulatory T cells actively down-regulate the immune response, the disparity between regulatory T cells and helper T cells tends to accelerate the course of the disease and is a strong clinical predictor of CD4+ depletion and death[xxxii].  The immunomodulatory effect of PRP could potentially help restore the balance of helper and regulatory T cells.

Material and Methods

Study Design

With this alternative treatment approach in mind, clinical trials were developed to test a new oral spray product containing colostrum-derived PRP as well as other growth and immune factors, including trypsin inhibitors, glycoconjugates, orotic acid, lysozyme, and others.  This was an open-label intervention/prospective trial to determine the effect of Cytolox® on HIV/AIDS patients.

Inclusion Criteria

Patients were eligible to participate in the study if they met the following criteria:

1) HIV seropostive
2) Symptomatic or asymptomatic
3) 18 years or older
4) Willing to maintain a proper diet and drink at least 5-8 8 oz. glasses of water a day
5) Be willing and able to take the Cytolox® PRP spray regularly, three sprays every four hours around the clock
6) Report for weekly follow-up visits to a physician for the duration of the study (30 days)

Potential participants were excluded if they had been treated with any other investigational drug(s) within the 30 day period prior to the onset of the study.  Women who were or thought they might become pregnant during the study were also excluded.

Dosage

Three sprays (2 ml total) of Cytolox® were given every four hours.

Measurements

Patients received an initial evaluation to assess the severity of the disease, have their weight measured, and blood drawn to measure baseline CD4+ counts.  A Symptom Assessment Form was completed to evaluate clinical symptoms, including diarrhea, nausea, vomiting, fever, cough, tuberculosis, fatigue/malaise, paresthesia, and any other symptoms.  Patients then returned to the clinic weekly for follow-up assessments and measurements, including the Symptom Assessment Form.  Physical findings included weight, chest exam, liver or spleen enlargement, lymphadenopathy, hair changes, and the presence or absence of skin rash or herpes zoster.  Lab tests were performed for CD4+ lymphocyte counts, white and red blood cell counts, hemoglobin, hematocrit, sed rate, and chest X-ray (if deemed necessary).  A record was also kept of other medications the patient was receiving.  Patients were also requested to report any adverse reactions they might experience.  Patients were also requested to sign an informed consent form.

At the end of the trial period, patients were allowed to continue or discontinue use of the spray at their or their physician’s discretion.  Patients were discontinued from the study if they failed to comply with taking the test product, they had an adverse reaction that was unacceptable to them or their physician, if their physician decided it was in the patient’s best interest, or if the patient wished to discontinue for any reason.

Safety
As Cytolox® is a food substance derived from colostrum rather than a drug, it is entirely safe for human consumption.  There are no known side effects nor drug interactions.  While those with lactose intolerance could potentially suffer an allergic reaction, such occurrences are exceedingly rare.

Results

Phase I trials were conducted at the Infectious Disease Clinic in Dayton, Ohio, from February to April, 1996.  Phase II trials were conducted at the University of Nairobi, Nairobi, Kenya, and in Nigeria from March to August 2000.  A total of 39 patients took part in the two studies.  Results of the Nigerian study are summarized in Tables 1-4.


Initial 30 Day 60 Day 90 Day
Total Patient Reports 35 31 20 17
Score 6.1 1.8 1.2 1.3
Percent Reduction
69 80 79
Expected Phase III  % Reduction
50-70 60-80 75-85

 

Table1.  Clinical Symptoms Score.


Initial 30 Day 60 Day 90 Day
Total Patient Reports 30 27 13 13
Score 4.0 2.5 2.1 1.6
Percent Reduction
38 49 60
Expected Phase III % Reduction
30-50 40-60 50-70

 

Table 2.  Physical Findings Score.



Initial Total 30 Day Total 60 Day Total

92,448 9,755 445

28,049 625 n/a

33,093 239 n/a

439 n/a 175

59,821 n/a 320

40,381 180 n/a
Expected Phase III % Reduction
<1,000 <500(<250 at 90 days)

 

Table 3.  Viral Load.  Viral load counts in six patients from the Phase II Trial.


Initial Total 30 Day Total 60 Day Total

74 153 121

274 282 n/a

245 301 n/a

60 47 n/a

101 n/a 117

211 n/a 291

249 271 n/a
Expected Phase III % Reduction
>250 >250(>500 at 90 days)

Table 4.  CD4+ Count.  Only available for seven patients from the Phase II Trial.

Tables 1 and 2 summarize the change in both clinical symptoms and physical findings of patients in the study.  Both show significant improvement over the 90 day period of the trial. Tables 3 and 4 show a dramatic decrease in viral load over 60 days and a concomitant increase in CD4+ lymphocyte counts over the same period. The status of specific clinical conditions in the patients was also monitored during the two studies.  Results are shown in Tables 5 and 6.

gdor-table5

Table 5.  Relief of diarrhea, nausea, vomiting, and fatigue/malaise in trial participants over the 90 days of the study.

gdor-table6

Table 6.  Relief of fever, cough, tuberculosis and paresthesia in trial participants over the 90 days of the study.

Both Tables 5 and 6 demonstrate a marked reduction in clinical symptoms over the 90 day period of the study.  Diarrhea, vomiting, cough, tuberculosis and paresthesia were eliminated in all test subjects, while nausea, fatigue and fever were also significantly reduced.

Tables 7, 8 and 9 summarize the improvement in CD4+ levels in patients over the course of the study.  Over half the participants had CD4+ counts in normal ranges by the conclusion of the study.  None had levels below 300, although prior to the study levels were well below normal (median 275).  All participants experienced increased CD4+ levels to some degree (Table 10).

A comment on the CD4+ results.  CD4+ counts are a valid marker of the progression of the HIV infection.  However, CD4+ levels are only one measure of wellness.  With PRP treatment, CD4+ levels are likely to normalize more slowly than other measures of wellness.  Viral load levels may actually increase in the peripheral blood after initiation of treatment as the virus is prevented from entering T cells, particularly in the lymph nodes.  This increase in blood levels of HIV causes a temporary drop in CD4+ levels in the peripheral blood.  CD4+ levels do increase over time with continued treatment.  Normal CD4+ counts for adults range from 500-1500 cells/mm3.

Finally, weight gains of up to 5% were recorded (Table 11).  Patients taking the PRP spray fared much better in terms of quality of life than did patients on anti-retroviral drugs.

Before After
No. % No. %
150-200 17 29% 0 0%
201-250 10 17% 0 0%
251-300 21 36% 0 0%
301-400 4 7% 8 14%
401-500 4 7% 8 14%
501-600 2 3% 12 21%
601-1000 0 0% 19 33%
Totals 58 58

Table 7 .  CD4+ counts in 58 experimental subjects before and after application of oral PRP spray.

gdor-table8

Table 8.  CD4+ lymphocyte levels in HIV compromised individuals before and after treatment with PRP oral spray.  This bar graph clearly illustrates the marked increase in CD4+ lymphocyte counts in patients with long-term HIV/AIDS and severely depleted CD4+ counts after administration of oral PRP spray.  Results from Trial 1 held in Nigeria.

gdor-table9

Table 9.  Cd4+ lymphocyte levels in HIV compromised individuals before and after treatment with PRP oral spray.  These results are from Trial 2 held in Kenya.

gdor-table10

Table 10.  A graphical representation of changes in CD4+ lymphocyte levels in patients participating in Trial 2.  While levels for some increased over 100% in some cases, what is particularly significant is that levels increased for all participants in the study.

gdor-table11

Table 11.  Weight loss/gain for patients on oral PRP or anti-retrovirus therapy.

Conclusions

The results of the African trials confirm the earlier results that an oral PRP spray treatment can be an important alternative or adjunct therapy for HIV/AIDS patients.  Further studies will be needed to study the long-term effects of the therapy and whether treatment over a longer period can eliminate the virus from the body.  Phase III trials are currently underway in India under the auspices of WHO, and results should soon be available.

In addition to Cytolox®, there are two similar products on the market, Immulox® (which is similar to Cytolox®) and Viralox®. Viralox® combines lactoferrin with the PRP.  Comparison trials with Viralox® and Cytolox® are scheduled in the near future in Africa.


[i] Figures cited in Wikipedia article on AIDS, http://en.wikipedia.org/wiki/AIDS

[ii] The Henry J. Kaiser Family Foundation: HIV/AIDS Policy Fact Sheet (November 2005).

[iii] United Nations Department of Economic and Social Affairs/Population Division report: The Impact of AIDS (2004).  http://www.un.org/esa/population/publications/AIDSimpact/AIDSWebAnnounce.htm

[v] U.S. Food and Drug Administration, “Drugs used in the treatment of HIV infection” (August 2006). http://www.fda.gov/oashi/aids/virals.html

[vi] Ghoneum, M. Enhancemant of Human Natural Killer Cell Activity by Modified Arabinoxylane from Rice Bran (MGN-3), INT. J. Immunotherapy XIV(2) 89-99 (1998).

[vii] Kelly, GS, , Larch Arabinogalctan: Clinical Relevance of a Novel Immune-enhancing polysaccharide. Altern Med Rev, 1999 Apr;4(2):96-103

[viii] "Patients HIV/AIDS treated with the homeopathic medicine Canova, they improve laboratory indexes, clinical and quality of life”. 2005 UFPR - Stroparo, E.

[ix] Glisodin study on oxidative stress in immune compromised humans” 2006 XVI International AIDS Conference. August 2006. Toronto, Canada.

[x] Natural Protection Against HIV/AIDS: “Natural Killers” Involved. "Increased NK Cell Activity in HIV-1 Exposed But Uninfected Vietnamese Intravascular Drug Users": The Journal of Immunology. 1 December 2003.

[xi] Andy Coghlan. "Mystery over drug trial debacle deepens", New Scientist, 2006-08-14. Retrieved on 2006-08-14.

[xii] Floren, CH, Chinenye, S, Elfstrand, L, Hagman, C, Ihse, I.  ColoPlus, a new product based on bovine colostrum, alleviates HIV-associated diarrhea. Scandinavian Journal of Gastroenterology 41(6):682-686 (2006).  HIV-associated diarrhea is common in AIDS patients in developing countries particularly.  It is a major contributory factor in the decrease in the helper CD4+ T-cell population.  ColoPlus is a product derived from bovine colostrum which is rich in immunoglobulins, growth factors, antibacterial peptides and nutrients.  It is specially designed for slow passage through the gastrointestinal tract and high nutritional value.  The study was undertaken to see if oral doses of ColoPlus could influence HIV-associated diarrhea.  The study was done in Nigeria and included 30 patients with HIV-associated diarrhea.  The patients were treated for 4 weeks in an open-labeled, non-randomized trial.  Results of the study showed a marked reduction in stool evacuations per day from 7±2.7 to 1.3±0.5, a decrease in self-estimated fatigue of 81%, an increase in body weight of 7.3 kg per patient, and a 125% increase in CD4+ count.

[xiii] Zimecki, M, Artym, J.  [Therapeutic properties of proteins and peptides from colostrum and milk] Post?py Higieny i Medycyny Doświadczalnej 59:309-323 (2005).  Colostrum and milk are rich in peptides and proteins which play an active role in innate immunity.  PRP has a variety of immunotropic functions, including the promotion of T cell maturation and inhibition of autoimmune disorders.

[xiv] Keech, A., Jimenez, R. “A Novel Colostral Fractionation Process”.  USPTO filing 2004, PCT 2005.

[xv] Keech, A. “ Novel Immunologically Active Peptide Fragments of a Proline-rich Polypeptide Isolated from Colostral Mammalian Fluids for Treatment of Viral and Non-Viral Diseases or Diseased Conditions”. USPTO Filing 2005, PCT 2006.

[xvi] Janusz, M, Staroscik, K, Zimecki, M, Wieczorek, Z, Lisowski, J. A proline-rich polypeptide (PRP) with immunoregulatory properties isolated from ovine colostrum. Murine thymocytes have on their surface a receptor specific for PRP. Archivum immunologiae et therapiae experimentalis (Warszava) 34(4):427-436 (1986).  PRP has immunoregulatory properties.  It induces the maturation of thymocytes into mature helper or suppressor T cells.

[xvii] Wieczorek, Z, Zimecki, M, Spiegel, K, Lisowski, J, Janusz, M.  Differentiation of T cells into helper cells from immature precursors: identification of a target cell for a proline-rich polypeptide (PRP). Archivum immunologiae et therapiae experimentalis (Warszava) 37(3-4):313-322 (1989).  The precursors of helper T cells belong to a minor thymocyte subset bearing the Thy-1 +/-, H-2+, L3T4-, lyt 2-, CD3- phenotype.  PRP induced the production of antigens consistent with mature helper T cells

[xviii] Bishop, GA, Haxhinasto, SA, Stunz, LL, Hostager, BS.  Antigen-specific B-lymphocyte activation. Critical Reviews in Immunology 23(3):159-197 (2003).  B cells have the exclusive ability to produce and secrete immunoglobulins of various types.  They also function in antigen presentation and the production of a number of cytokines and chemokines.

[xix] Shi, M, Hao, S, Chan, T, Xiang, J.  CD4+ T cells stimulate memory CD8+ T cell expansion via acquired pMHC I complexes and costimulatory molecules, and IL-2 secretion. Journal of Leukocyte Biology (2006).  CD8+ memory T cell expansion following a second encounter with a pathogen is a hallmark of adaptive immunity.  Antigen-specific CD4+ cells, activated by dendritic cells, stimulate the this expansion of CD8+ cells.

[xx] Zimecki, M, Staroscik, K, Janusz, M, Lisowski, J, Wieczorek, Z.  The inhibitory activity of a proline-rich polypeptide (PRP) on the immune response to polyvinylpyrrolidone (PVP). Archivum immunologiae et therapiae experimentalis (Warszava) 31(6):895-903 (1983).  PRP administered to a test animal before immunization with PVP inhibits the immune response to this antigen.  PRP did this by increasing the activity of suppressor T cells and by increasing the generation of new suppressor T cells.

[xxi] Julius, MH, Janusz, M, Lisowski, J.  A colostral protein that induces the growth and differentiation of resting B lymphocytes. Journal of Immunology 140(5):1366-371 (1988).  PRP induced resting B cells and supported their progression through the cell cycle to form mature B cells.  It had the same action on splenocytes.

[xxii] Kubis, A, Marcinkowska, E, Janusz, M, Lisowski, J.  Studies on the mechanism of action of a proline-rich polypeptide complex (PRP): effect on the stage of cell differentiation. Peptides 26(11):2188-2192 (2005).  PRP affects the differentiation and maturation of cells of the monocyte/ macrophage lineage and may regulate in this way the inflammatory processes in which these cells participate.

[xxiii] Research study carried out by Advanced Protein Systems, Phoenix, AZ, in 1999.  Unpublished results showed an increase in activity of NK cells by up to 248%, 5 times greater than any of 200 other products tested.

[xxiv] Effects of Oral Dietary Supplementation with Ai/E¹º® Upon Natural Killer (NK) Cell Activity in a Healthy Human Population. Quantum Research, Inc., Scottsdale, Arizona (2001).  Dialyzable Leukocyte Extract (DLE) was administered to 12 healthy male and female subjects aged 24-63.  Natural Killer (NK) cell activity was prior to initiation of the study and after completion of the study.  NK cell activity averaged 30 lytic units (LU) prior to the study and 101 LU following the study for an average increase of 207%.

[xxv] An Examination of Immune Response Modulation in Humans by Ai/E¹º® Utilizing A Double Blind Study. Immune Consultants, Inc., Tucson, Arizona (2001).  20 subjects, 10 men and 10 women, ranging in age from 32-61 participated in a double blind study in which 10 received DLE and the other 10 received placebo.  7 of the 10 receiving the DLE had a significant increase in three major immune markers: NK cell activity, TNF-α levels, and phagocytic index (PI), an indicator of macrophage activity.  Those receiving placebo had mixed results.

[xxvi] Zablocka, A, Janusz, M, Rybka, K, Wirkus-Romanowska, I, Kupryszewski, G, Lisowski, J.  Cytokine-inducing activity of a proline-rich polypeptide complex (PRP) from ovine colostrum and its active nonapeptide fragment analogs. European Cytokine Network 12(3):462-467 (2001).  PRP induces the production of INF-γ, TNF-α, IL-6 and IL-10 in human whole blood cultures.

[xxvii] Boldogh, I, Liebenthal, D, Hughes, TK, Juelich, TL, Georgiades, JA, Kruzel, ML, Stanton, GJ.  Modulation of 4HNE-mediated signaling by proline-rich peptides from ovine colostrum. Journal of Molecular Neuroscience 20(2):125-134 (2003).  PRP, also known as colostrinin, induces mitogenic stimulation as well as a variety of cytokines in peripheral leukocytes.  It also possess antioxidant activity in pheochromocytoma (P12) cells, a cancer cell line used for in vitro studies.  PRP was shown to reduce the amount of 4HNE-protein adducts, reduce intracellular levels of reactive oxygen species, inhibit 4HNE-mediated glutathione depletion, and inhibit 4HNE-induced activation of the molecular signal cascade which results in the production of c-Jun N-terminal kinase (JNK) in P12 cells.  This shows that PRP acts as both an antioxidant and a molecular signaling device.

[xxviii] Khan, A.  Non-specificity of transfer factor. Annals of Allergy 38(5):320-322 (1977).

[xxix] Fernandez-Ortega, C, Dubed, M, Ruibal, O, Vilarrubia, OL, Menendez de San Pedro, JC, Navea, L, Ojeda, M, Arana, MJ.  Inhibition of in vitro HIV infection by dialysable leucocyte extracts. Biotherapy 9(1-3):33-40 (1996).  A PRP extract from leukocytes inhibits HIV infection in MT-4 cell cultures.

[xxx] Raise, E, Guerra, L, Viza, D, Pizza, G, De Vinci, C, Schiattone, ML, Rocaccio, L, Cicognani, M, Gritti, F.  Preliminary results in HIV-1-infected patients treated with transfer factor (TF) and zidovudine (ZDV). Biotherapy 9(1-3):49-54 (1996).  HIV-1 specific transfer factor (an alternative name for PRP) plus zidovudine (ZDV) was tested for efficacy in patients with AIDS-related complex (ARC).  Patients receiving both transfer factor and ZDV experienced an increase in white blood cells, CD8+ lymphocytes and IL-2 levels over those receiving ZDV alone.

[xxxi] Pizza, G, Chiodo, F, Colangeli, V, Gritti, F, Raise, E, Fudenberg, HH, De Vinci, C, Viza, D.  Preliminary observations using HIV-specific transfer factor in AIDS. Biotherapy 9(1-3):4-47 (1996).  25 HIV infected patients at various stages (CDC stages II-IV) were treated with HIV-specific transfer factor (PRP) for periods of 60-1870 days.  All patients were receiving antiviral treatment as well.  Clinical improvement or a stabilized clinical condition was observed in 20 of the 25, and 12 of 14 anergic patients showed restored delayed hypersensitivity reactions to recall antigens within 60 days.  Treatment was well-tolerated and appears beneficial to AIDS patients.

[xxxii] Eggena, MP, Barugahare, B, Jones, N, Okello, M, Mutalya, S, Kityo, C, Mugyenyi, P, Cao, H.  Depletion of regulatory T cells in HIV infection is associated with immune activation. Journal of Immunology 174(7):4407-4414 (2005).  Immune activation during chronic HIV infection is a strong clinical predictor of death and may mediate helper CD4+ T cell depletion.  Regulatory T cells actively down-regulate immune responses.  In a study using 81 Ugandan volunteers, it was found that depletion of regulatory T cells occurs at different rates than other CD4+ T cells, resulting in an increased regulator to helper ratio in many patients with advanced disease.  This skewing may contribute to T cell effector dysfunction.

† These statements have not been evaluated by the Food and Drug Administration. These products are not intended to diagnose, treat, cure, or prevent any disease.

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